Matrices listed below were spiked with certain level of TXB2 and the recovery rates were calculated by comparing the measured value to the expected amount of TXB2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
94
EDTA plasma(n=5)
86-103
96
heparin plasma(n=5)
86-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TXB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
99-105%
91-104%
EDTA plasma(n=5)
85-94%
83-96%
93-101%
heparin plasma(n=5)
81-100%
84-97%
85-100%
Sensitivity
< 0.938pg/ml
Notes
For Research Use Only
Antigen
TXB2
Applications
ELISA
Applications Notes
TXB2 ELISA Kit allows for the in vitro quantitative determination of TXB2 concentrations in serum, plasma, tissue homogenates and other biological fluids.