Sheep insulin-like growth factor 1,IGF-1 ELISA Kit

Mfr.No.

CSB-E13753Sh

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Sheep

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

Recovery

The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	95	89-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-103
1:2	Average %	89
	Range %	83-99
1:4	Average %	95
	Range %	90-101
1:8	Average %	89
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P10763

Applications	ELISA
Research Area	IGF1

Storage	Store at 2-8°C. Please refer to protocol.

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