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Home / ELISA Kit / PharmaTest ELISA Kits

SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) (spike protein/ ACE2 ligand binding assay) COVID-19 sVNT

SKU : BHE18300017

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Mfr.No.	EL-1611-32214
Reactivity	Human
Assay Duration	2.5 hours
Assay Type	Competitive
Sample Volume	40 uL
Description	Neutralizing antibodies develop in some patients to varying degrees following infection with SARS-CoV-2 Reagent preparation Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. Results calculation 1. Neutralizing antibodies will vary in terms of concentration and affinity, this assay provides a qualitative readout. As such the user should use the comparable positive controls inhibitory controls when comparing interassay results. The provided controls are tested for comparability between lots and can be traced. 2. The protein titers in the test samples will fall in the range of high, medium, low or negative. We recommend each lab develop their own statistical cutpoint using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the validation of immunoassays used for detection of host antibodies against biotechnology products. J. Pharmaceutical and Biomedical Analysis 48:12671281). 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor. Sample preparation Samples are used neat.
Detection Method	Peroxidase / OD450
Kit Components	Coated microtiter plate, 96 wells Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition 10X wash buffer - 50ml Assay buffer - 50ml 1000X detection reagent - 150 ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Sample Type	Serum, Plasma
Components	Coated microtiter plate, 96 wells Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition 10X wash buffer - 50ml Assay buffer - 50ml 1000X detection reagent - 150 ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Precision	Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
Notes	This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
Usage Notes	Quantification of neutralizing antibodies
Protocol	SARS-CoV-2 spike protein ligand binding assay allows rapid quantification of COIVD19 neutralizing antibodies in serum that block the interaction between the viral spike protein and its target the ACE2 receptor.

Applications	ELISA

Storage	-20C, 1 year

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SKU	Supplier No.	Size	Your price	Quantity
BHE18300017	EL-1611-32214	1X96 wells	$1100.99		Add to Cart

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