Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Mfr.No.

EK1564

Sensitivity

<10 pg/ml

Detection Range

23.4 pg/ml - 1500 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	23.4	46.9	93.8	187.5	375	750	1500
O.D.	0.161	0.207	0.211	0.303	0.440	0.729	1.266	2.369

Description

Rat WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Wnt inhibitory factor 1;WIF-1;Wif1;

Molecular Weight

41585 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G29-W379

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat WIF1

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	46	179	730	48	172	703
Standard deviation	3.49	10.2	32.85	4.41	10.32	44.28
CV (%)	7.6%	5.7%	4.5%	9.2%	6%	6.3%

Protein Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Background

Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	46	51	47	44	47	2.54	5.4%
Sample 2	179	201	178	187	186	9.2	4.9%
Sample 3	730	656	670	709	691	29.62	4.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	114557
Antigen	WIF1/Wnt
Gene Name	Wif1
UniProt ID	Q6IN38
Protein Name	Wnt inhibitory factor 1

Applications	ELISA
Research Area	Secreted,Signaling Pathways,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001135	EK1564	96 wells/kit	$499		Add to Cart

Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

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