Rat VE-Cadherin Cdh5 ELISA Kit PicoKine®

Mfr.No.

EK1916

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.012	0.147	0.264	0.463	0.819	1.613	2.165	2.793

Description

Rat VE-Cadherin Cdh5 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cdh5 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin 5; Cdh5

Immunogen

Expression system for standard: NS0; Immunogen sequence: D46 - Q599

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat VE-Cadherin/CD144

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	399	1935	8758	443	2194	8947
Standard deviation	21.95	123.84	507.96	27.47	112.11	653.13
CV (%)	5.5%	6.4%	5.8%	6.2%	5.1%	7.3%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins.

Background

Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cellcell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	399	410	443	467	429	26.92	6.2%
Sample 2	1935	2194	2013	2122	2066	99.36	4.8%
Sample 3	8758	8677	8447	8601	8620	114.65	1.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	307618
Gene Name	Cdh5
UniProt ID	F1M7E5

Applications	ELISA
Research Area	Atherosclerosis,Cadherins,Cancer,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cytoskeleton/ECM,Invasion/Microenvironment,Signal Transduction,Vascular Inflammation,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001439	EK1916	96 wells/kit	$499		Add to Cart

Rat VE-Cadherin Cdh5 ELISA Kit PicoKine®

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