Rat Vascular Endothelial Cell Growth Factor D

Mfr.No.

E0662Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Vascular Endothelial Cell Growth Factor D (also known as VEGF-D) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

c-Fos-induced growth factor;FIGF;Vascular endothelial growth factor D;VEGF D;VEGFD;VEGF-D

Reactivity

Rat

Detection Range

0.1-40ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

37113

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.053ng/mL

Background

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor (By similarity).

Gene ID	360457
UniProt ID	O35251
Antigen	VEGFD

Research Area

Cardiovascular

Protocol

These standard curves of E0662Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.099	2.156	2.116
	2.214
12ng/mL	1.395	1.331	1.291
	1.267
6ng/mL	0.76	0.759	0.719
	0.758
3ng/mL	0.293	0.331	0.291
	0.369
1.5ng/mL	0.19	0.168	0.128
	0.146
0ng/mL	0.033	0.04	0
	0.047

Precision

We measured random samples of E0662Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.9	0.43	5.4
Intra-Assay	2	18	11.3	0.41	3.6
Intra-Assay	3	18	6	0.36	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111929	E0662Ra	48T	$320		Add to Cart
BHE12111929	E0662Ra	96T	$458		Add to Cart

Rat Vascular Endothelial Cell Growth Factor D - VEGF-D ELISA Kit

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