Rat Tryptase - TPS ELISA Kit

Mfr.No.

E0427Ra

Sensitivity

0.022ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Tryptase (also known as TPS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mast cell protease 7;rMCP-7;TPSAB 1;TPSAB1;Tryptase;Tryptase alpha/beta-1;Tryptase, skin

Molecular Weight

30400

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0427Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.7	0.13	7.6
Intra-Assay	2	18	4.1	0.27	6.5
Intra-Assay	3	18	0.6	0.03	4.8

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).

Protocol

These standard curves of E0427Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.108	2.085	2.029
	2.062
3.2ng/ml	1.713	1.718	1.662
	1.724
1.6ng/ml	1.166	1.134	1.078
	1.102
0.8ng/ml	0.673	0.678	0.622
	0.683
0.4ng/ml	0.43	0.434	0.378
	0.439
0ng/ml	0.065	0.056	0
	0.048

Gene ID	54271
UniProt ID	P27435

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111719	E0427Ra	48T	$320		Add to Cart
BHE12111719	E0427Ra	96T	$458		Add to Cart

Rat Tryptase - TPS ELISA Kit

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