Rat Tryptase Beta-2 - TPSB2 ELISA Kit

Mfr.No.

E2478Ra

Sensitivity

0.017ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Mast cell protease 6;Mcpt6;rMCP-6;TPSB2;Tryptase beta-2;Tryptase-2

Molecular Weight

30508

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2478Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.33	0.09	6.55
Intra-Assay	2	18	2.9	0.16	5.48
Intra-Assay	3	18	0.72	0.05	6.8

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity.

Protocol

These standard curves of E2478Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.052	2.141	2.103
	2.23
3.2ng/ml	1.801	1.768	1.729
	1.734
1.6ng/ml	1.235	1.177	1.138
	1.118
0.8ng/ml	0.664	0.673	0.635
	0.683
0.4ng/ml	0.426	0.412	0.373
	0.398
0ng/ml	0.041	0.038	0
	0.035

Gene ID	29268
UniProt ID	P50343

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115433	E2478Ra	48T	$320		Add to Cart
BHE12115433	E2478Ra	96T	$458		Add to Cart

Rat Tryptase Beta-2 - TPSB2 ELISA Kit

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