Rat TrkA ELISA Kit PicoKine®

Mfr.No.

EK0847

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.006	0.074	0.146	0.252	0.509	0.948	1.353	1.903

Description

Rat TrkA ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ntrk1 in cell culture supeatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

High affinity nerve growth factor receptor;2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;Slow nerve growth factor receptor;p140-TrkA;Trk-A;Ntrk1;Trk, Trka;

Molecular Weight

87868 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A33-P418

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat TrkA

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Intealized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes.

Sample Type

Cell culture supeatants, Cell lysates

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	115	863	2241	117	796	2366
Standard deviation	4.14	27.62	87.4	4.8	35.82	87.54
CV(%)	3.6%	3.2	3.9%	4.1%	4.5%	3.7%

Protein Function

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. .

Background

Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected coeas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	115	123	127	129	124	2.72	2.2%
Sample 2	863	769	752	806	798	16.75	2.1%
Sample 3	2241	2249	2369	2477	2334	72.35	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	59109
Gene Name	NTRK1
UniProt ID	P35739
Protein Name	High affinity nerve growth factor receptor
Tissue Specificity	Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues.
Sequence	Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Applications	ELISA
Research Area	Cancer,Oncoproteins/Suppressors,Oncoproteins,Neurology Process,Neuroscience,Growth and Development

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000653	EK0847	96 wells/kit	$499		Add to Cart

Rat TrkA ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat TrkA ELISA Kit PicoKine®

Rate it now..!!