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Home / ELISA Kit

Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit

SKU : BHE13700164

The deduced protein contains 1,236 amino acids. RT-PCR ELISA detected moderate VCPIP1 expression in all adult and fetal tissues examined.Reassembly of the Golgi apparatus from membrane fragments after cell division requires the ATPases NSF and p97 (VCP), as well as the cofactor p47 (NSFL1C) and the receptor syntaxin-5 (STX5A). rat Vcip135 was essential for p97/p47-mediated membrane fusion and that Vcip135 bound to the p97/p47/Stx5a complex and dissociated it via p97-catalyzed ATP hydrolysis. Microinjection of antibodies against Vcip135 and p47 in rat kidney cells showed that Vcip135 and p47 were required for Golgi and endoplasmic reticulum assembly.a cycle of ubiquitination and VCIP135-mediated deubiquitination regulates Golgi membrane dynamics during mitosis.

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Mfr.No.	KTE100015
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit employs a two-site sandwich ELISA to quantitate VCP.
Alternative Names	VCP; IBMPFD; MGC131997; MGC148092; MGC8560; TERA; p97; transitional endoplasmic reticulum ATPase; yeast Cdc48p homolog
Detection Method	Colorimetric
Kit Components	Rat Transitional endoplasmic reticulum ATPase microplate Rat Transitional endoplasmic reticulum ATPase standard Rat Transitional endoplasmic reticulum ATPase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VCP. No significant cross-reactivity or interference between Rat VCP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced protein contains 1,236 amino acids. RT-PCR ELISA detected moderate VCPIP1 expression in all adult and fetal tissues examined.Reassembly of the Golgi apparatus from membrane fragments after cell division requires the ATPases NSF and p97 (VCP), as well as the cofactor p47 (NSFL1C) and the receptor syntaxin-5 (STX5A). rat Vcip135 was essential for p97/p47-mediated membrane fusion and that Vcip135 bound to the p97/p47/Stx5a complex and dissociated it via p97-catalyzed ATP hydrolysis. Microinjection of antibodies against Vcip135 and p47 in rat kidney cells showed that Vcip135 and p47 were required for Golgi and endoplasmic reticulum assembly.a cycle of ubiquitination and VCIP135-mediated deubiquitination regulates Golgi membrane dynamics during mitosis.
Notes	This Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit employs a two-site sandwich ELISA to quantitate VCP in samples. An antibody specific for VCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	116643

Applications	ELISA
Applications Notes	This Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit employs a two-site sandwich ELISA to quantitate VCP in samples. An antibody specific for VCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700164	KTE100015	48 T	$339		Add to Cart
BHE13700164	KTE100015	96 T	$589		Add to Cart
BHE13700164	KTE100015	96 Tx5	$2629		Add to Cart
BHE13700164	KTE100015	96 Tx50	$24569		Add to Cart

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