Rat TNFSF12/Tweak ELISA Kit PicoKine®

Mfr.No.

EK1177

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.005	0.029	0.083	0.223	0.426	0.772	1.381	2.005

Description

Rat TNFSF12/Tweak ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tnfsf12 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protein Prmt2 ;Tumor necrosis factor ligand superfamily member 12 ;Tnfsf12 ;Prmt2 ;

Molecular Weight

27381 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S94-H249

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat TNFSF12

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	228	507	28	218	506
Standard deviation	1.7	9.8	30.42	1.96	9.59	37.44
CV (%)	5.5%	4.3%	6%	7%	4.4%	7.4%

Background

Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	31	31	28	30	1.29	4.3%
Sample 2	228	219	215	203	216	8.98	4.1%
Sample 3	507	509	522	445	495	29.86	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	360548
Gene Name	TNFSF12
UniProt ID	Q6AYC1
Protein Name	Tumor necrosis factor ligand superfamily member 12

Applications	ELISA
Research Area	Apoptosis,Cancer,Cell Biology,Cell Death,Growth Factors,Invasion/Microenvironment

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000800	EK1177	96 wells/kit	$499		Add to Cart

Rat TNFSF12/Tweak ELISA Kit PicoKine®

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