Rat Tissue Inhibitors of Metalloproteinase 2

Mfr.No.

E0323Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Tissue Inhibitors of Metalloproteinase 2 (also known as TIMP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Metalloproteinase inhibitor 2;TIMP 2;TIMP2;TIMP-2;Tissue inhibitor of metalloproteinases 2

Reactivity

Rat

Detection Range

0.5-90ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

24356

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.25ng/ml

Background

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Gene ID	29543
UniProt ID	P30121
Antigen	TIMP2

Research Area

Cancer

Protocol

These standard curves of E0323Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.107	2.191	2.163
	2.275
24ng/ml	1.415	1.384	1.356
	1.353
12ng/ml	0.682	0.673	0.645
	0.664
6ng/ml	0.359	0.39	0.362
	0.421
3ng/ml	0.143	0.173	0.145
	0.204
0ng/ml	0.029	0.028	0
	0.027

Precision

We measured random samples of E0323Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	25.3	0.91	3.6
Intra-Assay	2	18	19.8	1.08	5.5
Intra-Assay	3	18	11.8	0.51	4.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111622	E0323Ra	48T	$320		Add to Cart
BHE12111622	E0323Ra	96T	$458		Add to Cart

Rat Tissue Inhibitors of Metalloproteinase 2 - TIMP-2 ELISA Kit

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