Rat Tissue Factor - TF ELISA Kit

Mfr.No.

E0794Ra

Sensitivity

0.051ng/ml

Detection Range

0.1-3ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Tissue Factor (also known as TF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD142;Coagulation factor III;TF;Tissue factor

Molecular Weight

33444

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0794Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.05	0.38	3.14
Intra-Assay	2	18	6.78	0.27	4.01
Intra-Assay	3	18	3.41	0.13	3.93

Background

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The (TF:VIIa) complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Protocol

These standard curves of E0794Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	1.707	1.719	1.679
	1.731
8ng/ml	1.031	1.003	0.963
	0.974
4ng/ml	0.464	0.438	0.398
	0.412
2ng/ml	0.26	0.252	0.213
	0.245
1ng/ml	0.063	0.073	0.033
	0.083
0ng/ml	0.031	0.04	0
	0.048

Gene ID	25584
UniProt ID	P42533

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112046	E0794Ra	48T	$320		Add to Cart
BHE12112046	E0794Ra	96T	$458		Add to Cart

Rat Tissue Factor - TF ELISA Kit

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