Rat TIMP-2 ELISA Kit PicoKine®

Mfr.No.

EK1302

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.028	0.056	0.118	0.205	0.402	0.849	1.345	1.970

Description

Rat TIMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Timp2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Metalloproteinase inhibitor 2;Tissue inhibitor of metalloproteinases 2;TIMP-2;Timp2;Timp-2;

Molecular Weight

24356 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C27-P220

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat TIMP-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	240	1342	4533	227	1463	4769
Standard deviation	10.56	68.44	339.97	10.21	95.09	443.51
CV (%)	4.4%	9.3%	7.5%	4.5%	6.5%	9.3%

Protein Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Background

TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	240	251	238	213	235	13.9	5.9%
Sample 2	1342	1286	1409	1375	1353	45.35	3.3%
Sample 3	4533	4407	4200	4479	4404	126.38	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29543
Gene Name	TIMP2
UniProt ID	P30121
Protein Name	Metalloproteinase inhibitor 2
Sequence	Belongs to the protease inhibitor I35 (TIMP) family.

Applications	ELISA
Research Area	Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis/Ubiquitin,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000894	EK1302	96 wells/kit	$499		Add to Cart

Rat TIMP-2 ELISA Kit PicoKine®

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