Rat Tbc1 Domain Family Member 4

Mfr.No.

E1649Ra

Sensitivity

2.73ng/L

Detection Range

5-1000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat TBC1 Domain Family Member 4 (also known as TBC1D4/AS160) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Spermatogenesis-related factor 2;SRF-2;TBC1 domain family member 14;TBC1D14;Up-regulated in nephrectomized rat kidney #2

Molecular Weight

78209

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1649Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	193.2	10.57	5.5
Intra-Assay	2	18	511.6	20.27	4
Intra-Assay	3	18	87.3	5.06	5.8

Background

Plays a role in the regulation of starvation-induced autophagosome formation. Together with the TRAPPIII complex, regulates a constitutive trafficking step from peripheral recycling endosomes to the early Golgi, maintaining the cycling pool of ATG9 required for initiation of autophagy.

Protocol

These standard curves of E1649Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.249	2.168	2.127
	2.087
320ng/L	1.225	1.219	1.178
	1.213
160ng/L	0.857	0.859	0.817
	0.861
80ng/L	0.396	0.393	0.351
	0.39
40ng/L	0.243	0.231	0.19
	0.219
0ng/L	0.043	0.041	0
	0.04

Gene ID	360956
UniProt ID	Q5CD77

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112855	E1649Ra	48T	$320		Add to Cart
BHE12112855	E1649Ra	96T	$458		Add to Cart

Rat Tbc1 Domain Family Member 4 - TBC1D4 ELISA Kit

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