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Rat Steroid 21-hydroxylase - CYP21A2 ELISA Kit

BHE12112732

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

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Gene ID 24298
UniProt ID Q64562
Antigen CYP21A2
Research Area Cardiovascular
Protocol These standard curves of E1500Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.342 2.342 2.309
2.343
1600ng/L 1.354 1.443 1.409
1.532
800ng/L 0.815 0.821 0.788
0.828
400ng/L 0.424 0.442 0.408
0.46
200ng/L 0.294 0.257 0.223
0.22
0ng/L 0.033 0.034 0
0.034
Precision We measured random samples of E1500Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1530.18 52.24 3.41
Intra-Assay 2 18 285.14 13.29 4.66
Intra-Assay 3 18 697.45 32.92 4.72
Storage 2-8°C
SKU Supplier No. Size Your price Quantity
BHE12112732 E1500Ra 48T $320
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BHE12112732 E1500Ra 96T $458
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