Rat sL-Selectin ELISA Kit PicoKine®

Mfr.No.

EK0937

Sensitivity

<300 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	625	2000000	4000000	200000	400000	20000	40000
O.D.	0.004	0.550	0.919	1.411	1.799	2.074	2.129	2.226

Description

Rat sL-Selectin ELISA Kit PicoKine® (96 Tests). Quantitate Rat SELL in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

L-selectin ;Sell ;rCG_45999 ;

Molecular Weight

42471 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: W39-N332

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat sL-Selectin

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1474	3614	24600	1588	3716	23760
Standard deviation	89.91	166.24	1279.2	100.04	178.36	1663.2
CV (%)	6.1%	4.6%	5.2%	4.8%%	4.8%	7%

Protein Function

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Background

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1474	1304	1342	1320	1360	67.18	4.9%
Sample 2	3614	3452	3351	3314	3432	116.19	3.3%
Sample 3	24600	22997	23584	22075	23314	916.82	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29259
Gene Name	SELL
UniProt ID	Q63762
Protein Name	L-selectin
Sequence	Belongs to the selectin/LECAM family.

Applications	ELISA
Research Area	Adaptive Immunity,Cardiovascular,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells,T Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000710	EK0937	96 wells/kit	$499		Add to Cart

Rat sL-Selectin ELISA Kit PicoKine®

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