Rat SEZ6L ELISA Kit PicoKine®

Mfr.No.

EK1832

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.052	0.164	0.223	0.316	0.552	0.938	1.413	2.174

Description

Rat SEZ6L ELISA Kit PicoKine® (96 Tests). Quantitate Rat Sez6l in cell culture supeatants, serum and plasma (heparin). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Seizure-related 6 homolog-like; Sez6l

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-A958

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat SEZ6L

Subcellular Localization

Endoplasmic reticulum. Integral component of membrane. Neuronal cell body.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	176	807	4126	260	941	4859
Standard deviation	8.62	34.70	169.17	15.60	54.58	335.27
CV (%)	4.9%	4.3%	4.1%	6.0%	5.8%	6.9%

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. It is mapped to 12q16.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	176	199	178	190	185	9.33	5.0%
Sample 2	807	825	758	743	783	33.78	4.3%
Sample 3	4126	3875	3712	3928	3910	147.82	3.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	304554
Gene Name	Sez6l
UniProt ID	D4AD89

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001611	EK1832	96 wells/kit	$750		Add to Cart

Rat SEZ6L ELISA Kit PicoKine®

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