Rat Serpin A12 ELISA Kit PicoKine®

Mfr.No.

EK1741

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.087	0.171	0.295	0.574	1.100	1.981	2.761

Description

Rat Serpin A12 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Serpina12 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; Serpina12

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-P411

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Serpin A12

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	18	79	426	25	86	439
Standard deviation	0.86	4.50	19.60	1.28	5.07	28.10
CV (%)	4.8%	5.7%	4.6%	5.1%	5.9%	6.4%

Protein Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Background

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	18	19	17	16	17	1.11	6.5%
Sample 2	79	82	78	92	82	5.53	6.7%
Sample 3	426	461	468	504	464	27.68	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene Name	Serpina12
UniProt ID	Q8R4Z1
Tissue Specificity	Expressed in visceral adipose tissues.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001597	EK1741	96 wells/kit	$499		Add to Cart

Rat Serpin A12 ELISA Kit PicoKine®

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