Rat Semaphorin-3A - SEMA3A ELISA Kit

Mfr.No.

E2251Ra

Alternative Names

Sema III;SEMA3A;Semaphorin III;Semaphorin-3A

Reactivity

Rat

Detection Range

3-900ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

88808

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.55ng/mL

Background

May be involved in guiding growing axons towards their targets by forming a molecular boundary that instructs axons to engage in the formation of specific nerve tracts. Binds to neuropilin. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).

Gene ID	29751
UniProt ID	Q63548
Antigen	SEMA3A

Research Area

Neuroscience

Protocol

These standard curves of E2251Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/mL	2.151	2.114	2.078
	2.077
240ng/mL	1.636	1.647	1.611
	1.658
120ng/mL	0.97	0.99	0.953
	1.01
60ng/mL	0.682	0.715	0.679
	0.748
30ng/mL	0.495	0.462	0.425
	0.428
0ng/mL	0.046	0.036	0
	0.027

Precision

We measured random samples of E2251Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	240.03	14.58	6.07
Intra-Assay	2	18	108.79	8.56	7.87
Intra-Assay	3	18	65.05	5.83	8.97

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113383	E2251Ra	48T	$320		Add to Cart
BHE12113383	E2251Ra	96T	$458		Add to Cart

Rat Semaphorin-3A - SEMA3A ELISA Kit

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