Rat SCGB3A1 ELISA Kit PicoKine®

Mfr.No.

EK1994

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.058	0.137	0.164	0.321	0.520	0.842	1.348	2.081

Description

Rat SCGB3A1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat SCGB3A1 in cell culture supeatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Secretoglobin, family 3A, member 1; Scgb3a1

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-G104

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat SCGB3A1

Subcellular Localization

Extracellular region. Extracellular space.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	30	176	389	31	184	406
Standard deviation	1.41	9.15	22.17	1.95	9.94	28.83
CV (%)	4.7%	5.2%	5.7%	6.3%	5.4%	7.1%

Background

Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	30	34	33	32	32	1.47	4.5%
Sample 2	176	163	162	173	168	6.10	3.6%
Sample 3	389	365	413	429	399	24.24	6.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	338418
Gene Name	SCGB3A1
UniProt ID	E9PU36

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Cytokines,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001505	EK1994	96 wells/kit	$750		Add to Cart

Rat SCGB3A1 ELISA Kit PicoKine®

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