Matrices listed below were spiked with certain level of RETN and the recovery rates were calculated by comparing the measured value to the expected amount of RETN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
97-104
99
EDTA plasma(n=5)
88-102
94
heparin plasma(n=5)
88-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RETN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RETN ELISA Kit allows for the in vitro quantitative determination of RETN concentrations in serum, plasma, tissue homogenates and other biological fluids.