Rat Proenkephalin-B - PDYN ELISA Kit

Mfr.No.

E2210Ra

Alternative Names

Alpha-neoendorphin;Beta-neoendorphin;Beta-neoendorphin-dynorphin;Big Dyn;Big dynorphin;Dyn-A17;Dyn-B;Dynorphin A;Dynorphin A(1-13;Dynorphin A(1-17;Dynorphin A(1-8;Dynorphin B;Dynorphin B(1-13;Dynorphin B-29);Leu-enkephalin;Leumorphin;PDYN;Preprodynorphin;Proenkephalin-B;Rimorphin

Reactivity

Rat

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28079

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.27ng/L

Background

Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (By similarity). Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin (By similarity). Leumorphin has a typical opiod activity and may have anti-apoptotic effect.

Gene ID	29190
UniProt ID	P06300
Antigen	PDYN

Research Area

Others

Protocol

These standard curves of E2210Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.307	2.22	2.185
	2.134
600ng/L	1.479	1.552	1.517
	1.625
300ng/L	1.018	1.091	1.056
	1.164
150ng/L	0.648	0.646	0.611
	0.643
75ng/L	0.411	0.409	0.374
	0.407
0ng/L	0.034	0.035	0
	0.036

Precision

We measured random samples of E2210Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	99.18	8.48	8.55
Intra-Assay	2	18	206.42	12.41	6.01
Intra-Assay	3	18	475.23	22.86	4.81

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113342	E2210Ra	48T	$320		Add to Cart
BHE12113342	E2210Ra	96T	$458		Add to Cart

Rat Proenkephalin-B - PDYN ELISA Kit

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