Rat Phosphatidylinositol-glycan-specific Phospholipase D

Mfr.No.

E1439Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Phosphatidylinositol-glycan-specific phospholipase D (also known as GPLD1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Glycoprotein phospholipase D;Glycosyl-phosphatidylinositol-specific phospholipase D;GPI-PLD;GPI-specific phospholipase D;GPLD 1;GPLD1;Phosphatidylinositol-glycan-specific phospholipase D;PI-G PLD

Reactivity

Rat

Detection Range

0.5-200ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

93782

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.24ng/ml

Background

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.

Gene ID	291132
UniProt ID	Q8R2H5
Antigen	GPLD1

Research Area

Others

Protocol

These standard curves of E1439Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.101	2.108	2.052
	2.116
60ng/ml	1.866	1.891	1.835
	1.917
30ng/ml	1.13	1.126	1.069
	1.122
15ng/ml	0.711	0.707	0.65
	0.702
7.5ng/ml	0.439	0.409	0.353
	0.379
0ng/ml	0.066	0.056	0
	0.047

Precision

We measured random samples of E1439Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	27.6	1.78	6.4
Intra-Assay	2	18	42.6	1.8	4.2
Intra-Assay	3	18	14.4	0.76	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112672	E1439Ra	48T	$320		Add to Cart
BHE12112672	E1439Ra	96T	$458		Add to Cart

Rat Phosphatidylinositol-glycan-specific Phospholipase D - GPLD1 ELISA Kit

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