Matrices listed below were spiked with certain level of Pfkl and the recovery rates were calculated by comparing the measured value to the expected amount of Pfkl in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-105
98
EDTA plasma(n=5)
88-102
93
heparin plasma(n=5)
86-97
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pfkl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Pfkl ELISA Kit allows for the in vitro quantitative determination of Pfkl concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Pfkl(6-phosphofructokinase, liver type) ELISA Kit