Rat PDGF-AB ELISA Kit PicoKine®

Mfr.No.

EK0485

Sensitivity

<3 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.022	0.097	0.135	0.241	0.470	0.832	1.349	1.833

Description

Rat PDGF-AB ELISA Kit PicoKine® (96 Tests). Quantitate Rat PDGF-AB in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Platelet derived growth factor, B polypeptide ;Platelet-derived growth factor subunit B ;Pdgfb ;rCG_59494 ;

Molecular Weight

27422 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat PDGF-AB

Subcellular Localization

Pdgfa: Secreted. Note: Released by platelets uponwounding.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	86	282	885	83	277	930
Standard deviation	4.9	14.38	58.41	4.98	16.89	74.4
CV (%)	5.7%	8%%	6.6%	6%	6.1%	8%

Protein Function

Pdgfa: Growth factor that plays an essential role in theregulation of embryonic development, cell proliferation, cellmigration, survival and chemotaxis. Potent mitogen for cells ofmesenchymal origin. Required for normal lung alveolar septumformation during embryogenesis, normal development of thegastrointestinal tract, normal development of Leydig cells andspermatogenesis. Required for normal oligodendrocyte developmentand normal myelination in the spinal cord and cerebellum. Plays animportant role in wound healing. Signaling is modulated by theformation of heterodimers with PDGFB (By similarity).

Background

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	86	83	85	86	85	1.22	1.4%
Sample 2	282	277	283	282	281	2.34	0.8%
Sample 3	885	834	857	888	866	22.07	2.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24628, 25266
Gene Name	PDGFB, PDGFA
UniProt ID	P28576, Q05028
Protein Name	Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Immunology,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Pathways and Processes,Platelets,Response To Hypoxia,Secreted Molecules,Serum Proteins,Signal Transduction,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000475	EK0485	96 wells/kit	$499		Add to Cart

Rat PDGF-AB ELISA Kit PicoKine®

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