Rat Paraoxonase-1 - PON1 ELISA Kit

Mfr.No.

E0867Ra

Sensitivity

0.53ng/ml

Detection Range

1-400ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Paraoxonase-1 (also known as PON1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A-esterase 1;Aromatic esterase 1;PON 1;PON1;Serum aryldialkylphosphatase 1;Serum paraoxonase/arylesterase 1

Molecular Weight

39358

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0867Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	114	6.28	5.5
Intra-Assay	2	18	38.3	1.78	4.6
Intra-Assay	3	18	33.3	1.86	5.6

Background

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Protocol

These standard curves of E0867Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.296	2.238	2.185
	2.18
120ng/ml	1.891	1.862	1.81
	1.834
60ng/ml	1.098	1.181	1.128
	1.263
30ng/ml	0.601	0.612	0.559
	0.623
15ng/ml	0.355	0.398	0.345
	0.441
0ng/ml	0.042	0.053	0
	0.063

Gene ID	84024
UniProt ID	P55159

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112118	E0867Ra	48T	$320		Add to Cart
BHE12112118	E0867Ra	96T	$458		Add to Cart

Rat Paraoxonase-1 - PON1 ELISA Kit

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