Matrices listed below were spiked with certain level of PAPPA and the recovery rates were calculated by comparing the measured value to the expected amount of PAPPA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
97
EDTA plasma(n=5)
89-104
97
heparin plasma(n=5)
90-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAPPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PAPP-A(Pappalysin-1)/PAPPA/PAPA/DIPLA1/IGFBP-4ase/PAPPA1/ASBABP2/Pappalysin 1/Pregnancy Associated Plasma Protein A
Notes
For Research Use Only
UniProt ID
D3ZNQ7
Antigen
PAPPA
Applications
ELISA
Applications Notes
PAPPA ELISA Kit allows for the in vitro quantitative determination of PAPPA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit