Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine®

Mfr.No.

EK1594

Sensitivity

<5 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.094	0.224	0.324	0.514	0.748	1.063	1.533	2.078

Description

Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat CDH3 in cell culture supeatants and serum. Sensitivity: 5pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin 3

Immunogen

Expression system for standard: NS0; Immunogen sequence: E100-G647

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat P-Cadherin

Sample Type

Cell culture supeatants, Serum

Anticoagulant

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	28	221	384	30	229	407
Standard deviation	1.18	9.95	19.58	1.77	14.66	30.5
CV (%)	4.2%	4.5%	5.1%	5.9%	6.4%	7.5%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins.

Background

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	28	29	32	33	30	2.06	6.8%
Sample . 2	221	206	218	202	211	7.94	3.7%%
Sample 3	384	382	382	417	387	18.82	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	116777
Gene Name	CDH3
UniProt ID	F1LMI3

Applications	ELISA
Research Area	Cadherins,Cell Adhesion,Cytoskeleton/ECM,Neuroscience,Sensory System,Signal Transduction,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001378	EK1594	96 wells/kit	$499		Add to Cart

Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine®

Rate it now..!!