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Home / ELISA Kit

Rat Nuclear factor-kappa B (NF-kB) ELISA Kit

SKU : BHE13701055

Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappaB (NF-kappaB) protein complex.
NF-kappaB is a transcription factor that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NF-kappaB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions; over 200 known genes are targets of NF-kappaB in various cell types, under specific conditions.

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Mfr.No.	KTE100827
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1.
Alternative Names	NFKB1; DKFZp686C01211; EBP-1; KBF1; MGC54151; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; p105; p50; DNA binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA bi
Detection Method	Colorimetric
Kit Components	Rat Nuclear factor-kappa B microplate Rat Nuclear factor-kappa B standard Rat Nuclear factor-kappa B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Nuclear factor-kappa B (NF-kB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NFKB1. No significant cross-reactivity or interference between Rat NFKB1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappaB (NF-kappaB) protein complex. NF-kappaB is a transcription factor that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NF-kappaB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions; over 200 known genes are targets of NF-kappaB in various cell types, under specific conditions.
Notes	This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1 in samples. An antibody specific for NFKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1 in samples. An antibody specific for NFKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13701055	KTE100827	48 T	$359		Add to Cart
BHE13701055	KTE100827	96 T	$619		Add to Cart
BHE13701055	KTE100827	96 Tx5	$2809		Add to Cart
BHE13701055	KTE100827	96 Tx50	$26209		Add to Cart

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