Rat Noggin - NOG ELISA Kit

Mfr.No.

E0921Ra

Sensitivity

0.025ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Fragment;NOG;Noggin

Molecular Weight

16139

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0921Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.61	0.1	3.75
Intra-Assay	2	18	2.17	0.17	7.95
Intra-Assay	3	18	0.48	0.02	5.13

Background

Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (By similarity). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).

Protocol

These standard curves of E0921Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.303	2.287	2.252
	2.272
4ng/ml	1.487	1.602	1.567
	1.718
2ng/ml	1.102	1.114	1.078
	1.126
1ng/ml	0.689	0.66	0.624
	0.63
0.5ng/ml	0.43	0.449	0.414
	0.469
0ng/ml	0.032	0.035	0
	0.039

Gene ID	25495
UniProt ID	Q62809

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112172	E0921Ra	48T	$320		Add to Cart
BHE12112172	E0921Ra	96T	$458		Add to Cart

Rat Noggin - NOG ELISA Kit

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