Rat Neurotrophin 4 - NT-4 ELISA Kit

Mfr.No.

E0544Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Neurotrophin 4 (also known as NT-4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Neurotrophin-4;Neurotrophin-5;Neutrophic factor 4;NT-4;NT4P;NT-5;Ntf5

Reactivity

Rat

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22333

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.87ng/L

Background

Could serve as a target-derived trophic factor for sensory and sympathetic neurons.

Gene ID	25730
UniProt ID	P34131
Antigen	NT-4

Research Area

Neuroscience

Protocol

These standard curves of E0544Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.277	2.218	2.179
	2.158
1600ng/L	1.526	1.505	1.466
	1.484
800ng/L	1.006	1.032	0.993
	1.058
400ng/L	0.733	0.699	0.66
	0.665
200ng/L	0.467	0.47	0.431
	0.472
0ng/L	0.028	0.039	0
	0.05

Precision

We measured random samples of E0544Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1857.98	98.79	5.32
Intra-Assay	2	18	120.62	9.11	7.55
Intra-Assay	3	18	906.8	64.7	7.14

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111825	E0544Ra	48T	$320		Add to Cart
BHE12111825	E0544Ra	96T	$458		Add to Cart

Rat Neurotrophin 4 - NT-4 ELISA Kit

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