Rat Neurofilament Light Polypeptide

Mfr.No.

E1747Ra

Sensitivity

0.67ng/L

Detection Range

1-400ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Neurofilament Light Polypeptide (also known as NEFL) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

68 kDa neurofilament protein;NEFL;Neurofilament light polypeptide;Neurofilament triplet L protein;NF-L

Molecular Weight

61335

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1747Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	127.4	5.59	4.4
Intra-Assay	2	18	172.3	4.87	2.8
Intra-Assay	3	18	58	1.98	3.4

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Protocol

These standard curves of E1747Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.087	2.111	2.071
	2.136
120ng/L	1.102	1.13	1.089
	1.159
60ng/L	0.516	0.484	0.443
	0.453
30ng/L	0.192	0.222	0.181
	0.251
15ng/L	0.114	0.138	0.097
	0.162
0ng/L	0.056	0.041	0
	0.025

Gene ID	83613
UniProt ID	P19527

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112953	E1747Ra	48T	$320		Add to Cart
BHE12112953	E1747Ra	96T	$458		Add to Cart

Rat Neurofilament Light Polypeptide - NEFL ELISA Kit

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