Rat Neurofibromin - NF1 ELISA Kit

Mfr.No.

E2518Ra

Alternative Names

Neurofibromatosis-related protein NF-1;Neurofibromin;Nf1

Reactivity

Rat

Detection Range

20-3800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

317083

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

11.22ng/L

Background

Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity (By similarity).

Gene ID	24592
UniProt ID	P97526
Antigen	Nf1

Research Area

Neuroscience

Protocol

These standard curves of E2518Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.563	2.494	2.463
	2.425
1000ng/L	1.488	1.461	1.43
	1.434
500ng/L	0.935	0.851	0.82
	0.767
250ng/L	0.477	0.463	0.432
	0.449
125ng/L	0.2	0.201	0.17
	0.202
0ng/L	0.032	0.031	0
	0.029

Precision

We measured random samples of E2518Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	720.4	29.87	4.15
Intra-Assay	2	18	832.16	23.35	2.81
Intra-Assay	3	18	410.54	24.95	6.08

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115422	E2518Ra	48T	$320		Add to Cart
BHE12115422	E2518Ra	96T	$458		Add to Cart

Rat Neurofibromin - NF1 ELISA Kit

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