Rat Nephrin - NPHN ELISA Kit

Mfr.No.

E1366Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Nephrin (also known as NPHN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Nephrin;NPHS 1;NPHS1;Renal glomerulus-specific cell adhesion receptor

Reactivity

Rat

Detection Range

0.2-60ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

136281

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.109ng/ml

Background

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

Gene ID	64563
UniProt ID	Q9R044
Antigen	NPHS1

Research Area

Signal Transduction

Protocol

These standard curves of E1366Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.274	2.272	2.228
	2.271
20ng/ml	1.147	1.265	1.222
	1.383
10ng/ml	0.636	0.637	0.593
	0.637
5ng/ml	0.489	0.479	0.435
	0.469
2.5ng/ml	0.18	0.194	0.15
	0.208
0ng/ml	0.045	0.044	0
	0.042

Precision

We measured random samples of E1366Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.5	0.45	5.9
Intra-Assay	2	18	17.9	1.02	5.7
Intra-Assay	3	18	28.2	0.82	2.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112601	E1366Ra	48T	$320		Add to Cart
BHE12112601	E1366Ra	96T	$458		Add to Cart

Rat Nephrin - NPHN ELISA Kit

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