Matrices listed below were spiked with certain level of NEFL and the recovery rates were calculated by comparing the measured value to the expected amount of NEFL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
93
EDTA plasma(n=5)
86-104
94
heparin plasma(n=5)
92-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NEFL/NF-L/68 kDa neurofilament protein/Neurofilament triplet L protein
Notes
For Research Use Only
UniProt ID
P19527
Antigen
NEFL
Applications
ELISA
Applications Notes
NEFL ELISA Kit allows for the in vitro quantitative determination of NEFL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit