Rat Nadphoxidase 1 - NOX1 ELISA Kit

Mfr.No.

E1408Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat NADPH oxidase 1 (also known as NOX1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mitogenic oxidase 1;MOX-1;NADH/NADPH mitogenic oxidase subunit P65-MOX;NADPH oxidase 1;NOH-1;NOX 1;NOX1;NOX-1

Reactivity

Rat

Detection Range

70-15000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

65177

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

34.21ng/L

Background

Pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism.

Gene ID	114243
UniProt ID	Q9WV87
Antigen	NOX1

Research Area

Others

Protocol

These standard curves of E1408Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8000ng/L	2.373	2.28	2.233
	2.188
4000ng/L	1.783	1.829	1.781
	1.875
2000ng/L	1.272	1.213	1.166
	1.154
1000ng/L	0.699	0.665	0.617
	0.631
500ng/L	0.394	0.392	0.344
	0.389
0ng/L	0.031	0.048	0
	0.064

Precision

We measured random samples of E1408Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2027.8	138.78	6.8
Intra-Assay	2	18	3917.3	281.36	7.2
Intra-Assay	3	18	649.1	33.05	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112642	E1408Ra	48T	$320		Add to Cart
BHE12112642	E1408Ra	96T	$458		Add to Cart

Rat Nadphoxidase 1 - NOX1 ELISA Kit

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