Rat Nadph--cytochrome P450 Reductase

Mfr.No.

E1230Ra

Sensitivity

0.053ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat NADPH-Cytochrome P450 Reductase (also known as POR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CPR;CYPOR;NADPH--cytochrome P450 reductase;P450R;POR

Molecular Weight

76963

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1230Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.32	0.64	5.22
Intra-Assay	2	18	5.74	0.43	7.42
Intra-Assay	3	18	3.13	0.23	7.39

Background

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Protocol

These standard curves of E1230Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.298	2.268	2.206
	2.238
12ng/mL	1.728	1.743	1.681
	1.759
6ng/mL	1.1	1.121	1.059
	1.143
3ng/mL	0.663	0.697	0.635
	0.731
1.5ng/mL	0.353	0.397	0.334
	0.441
0ng/mL	0.058	0.062	0
	0.067

Gene ID	29441
UniProt ID	P00388

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112475	E1230Ra	48T	$320		Add to Cart
BHE12112475	E1230Ra	96T	$458		Add to Cart

Rat Nadph--cytochrome P450 Reductase - POR ELISA Kit

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