Rat Mothers Against Decapentaplegic Homolog 1

Mfr.No.

E0077Ra

Alternative Names

MAD homolog 1;Madh1;Mothers against decapentaplegic homolog 1;Mothers against DPP homolog 1;SMAD 1;SMAD family member 1;Smad1

Reactivity

Rat

Detection Range

8.12-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

52713

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.1ng/L

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). Has been shown to be also activated by TGF-beta (transforming growth factor) type I receptor kinase in rat intestinal epithelial cells (IEC 4-1). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene [removed]By similarity).

Gene ID	25671
UniProt ID	P97588
Antigen	Smad1

Research Area

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Protocol

These standard curves of E0077Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.236	2.164	2.104
	2.092
600ng/L	1.141	1.261	1.201
	1.38
300ng/L	0.856	0.774	0.714
	0.692
150ng/L	0.425	0.421	0.361
	0.417
75ng/L	0.228	0.215	0.155
	0.201
0ng/L	0.056	0.06	0
	0.063

Precision

We measured random samples of E0077Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	473.55	27.46	5.8
Intra-Assay	2	18	621.38	20.43	3.29
Intra-Assay	3	18	185.5	11.93	6.43

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111397	E0077Ra	48T	$320		Add to Cart
BHE12111397	E0077Ra	96T	$458		Add to Cart

Rat Mothers Against Decapentaplegic Homolog 1 - SMAD1 ELISA Kit

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