Rat Mitochondrial Uncoupling Protein 2

Mfr.No.

E1766Ra

Sensitivity

5.03ng/L

Detection Range

9-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Mitochondrial Uncoupling Protein 2 (also known as UCP2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mitochondrial uncoupling protein 2;Solute carrier family 25 member 8;UCP 2;UCP2

Molecular Weight

33377

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1766Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	815.9	30.6	3.8
Intra-Assay	2	18	273.9	12.87	4.7
Intra-Assay	3	18	377.6	13.75	3.6

Background

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).

Protocol

These standard curves of E1766Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.129	2.125	2.084
	2.12
600ng/L	1.517	1.495	1.455
	1.474
300ng/L	0.581	0.665	0.624
	0.749
150ng/L	0.322	0.369	0.329
	0.416
75ng/L	0.214	0.179	0.138
	0.144
0ng/L	0.038	0.04	0
	0.043

Gene ID	54315
UniProt ID	P56500

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112971	E1766Ra	48T	$320		Add to Cart
BHE12112971	E1766Ra	96T	$458		Add to Cart

Rat Mitochondrial Uncoupling Protein 2 - UCP2 ELISA Kit

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