Rat Mitochondrial Uncoupling Protein 2

Mfr.No.

E1766Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Mitochondrial Uncoupling Protein 2 (also known as UCP2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mitochondrial uncoupling protein 2;Solute carrier family 25 member 8;UCP 2;UCP2

Reactivity

Rat

Detection Range

9-2000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

33377

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.03ng/L

Background

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).

Gene ID	54315
UniProt ID	P56500
Antigen	UCP2

Research Area

Others

Protocol

These standard curves of E1766Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.129	2.125	2.084
	2.12
600ng/L	1.517	1.495	1.455
	1.474
300ng/L	0.581	0.665	0.624
	0.749
150ng/L	0.322	0.369	0.329
	0.416
75ng/L	0.214	0.179	0.138
	0.144
0ng/L	0.038	0.04	0
	0.043

Precision

We measured random samples of E1766Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	815.9	30.6	3.8
Intra-Assay	2	18	273.9	12.87	4.7
Intra-Assay	3	18	377.6	13.75	3.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112971	E1766Ra	48T	$320		Add to Cart
BHE12112971	E1766Ra	96T	$458		Add to Cart

Rat Mitochondrial Uncoupling Protein 2 - UCP2 ELISA Kit

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