Rat Microtubule-associated Protein 2

Mfr.No.

E1772Ra

Sensitivity

0.022ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Microtubule-associated Protein 2 (also known as MAP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

MAP 2;MAP2;MAP-2;Microtubule-associated protein 2

Molecular Weight

202411

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1772Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.9	0.1	2.5
Intra-Assay	2	18	3.4	0.12	3.7
Intra-Assay	3	18	1.6	0.08	4.7

Background

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Protocol

These standard curves of E1772Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	1.815	1.882	1.823
	1.949
3.2ng/ml	1.058	1.048	0.99
	1.038
1.6ng/ml	0.572	0.556	0.498
	0.54
0.8ng/ml	0.176	0.183	0.125
	0.191
0.4ng/ml	0.06	0.074	0.016
	0.088
0ng/ml	0.06	0.058	0
	0.057

Gene ID	25595
UniProt ID	P15146

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112975	E1772Ra	48T	$320		Add to Cart
BHE12112975	E1772Ra	96T	$458		Add to Cart

Rat Microtubule-associated Protein 2 - MAP-2 ELISA Kit

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