Rat Melatonin (MT)ELISA Kit

Rat Melatonin (MT)ELISA Kit

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Mfr.No.

CSB-E13433r

Sensitivity

12.5 pg/mL

Detection Range

12.5 pg/mL-500 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Melatonin (MT)

Recovery

The recovery of mouse CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	94	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	95
	Range %	90-100
1:400	Average %	87
	Range %	82-93
1:800	Average %	90
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.597	2.580	2.589	2.412
25	2.263	2.298	2.281	2.104
12.5	1.779	1.799	1.789	1.612
6.25	1.217	1.252	1.235	1.058
3.12	0.690	0.674	0.682	0.505
1.56	0.458	0.427	0.443	0.266
0.78	0.285	0.297	0.291	0.114
0	0.176	0.178	0.177	?

Species

Rattus norvegicus

Sample Type

Serum, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	MT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Protective effect of melatonin against inequality-induced damages on testicular tissue and sperm parameters Nasiraei-Moghadam SN et al,Int J Fertil Steril,2014 Jan;7(4):313-22. Different periods of intrauterine exposure to electromagnetic field: Influence on female rats' fertility, prenatal and postnatal development Alchalabi A S H.et al,Asian Pacific Journal of Reproduction,5(1): 14-23. Different periods of intrauterine exposure to electromagnetic field: Influence on female rats' fertility,prenatal and postnatal development Ali S.H. Alchalabi.et al,Asian Pacific Journal of Reproduction,2016 5(1):14-23 Effect of circadian rhythm disturbance on morphine preference and addiction in male rats: Involvement of period genes and dopamine D1 receptor Garmabi B. et al,Neuroscience.,2016 322:104-114. Evaluation of effects of melatonin and caffeic acid phenethyl ester on acute potassium dichromate toxicity and genotoxicity in rats Mujgan Cengiz.et al,Indian journal of pharmacology,48(4): 407-411 Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague-Dawley Rats Ali Saeed Al-Chalabi.et al,HAYATI Journal of Biosciences,Sep Cardioprotective Effects of HuoxueAnshen Recipe against Myocardial Injuries Induced by Sleep Deprivation in Rats Rong Yuan.et al,Evidence-Based Complementary and Alternative Medicine,5 April Thymulin, free or bound to PBCA nanoparticles, protects mice against chronic septic inflammation Elena G.et al,PLOS ONE,May 24

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.