Rat Malate Dehydrogenase - Cytoplasmic

Mfr.No.

E2140Ra

Sensitivity

1.355mU/mL

Detection Range

3-900mU/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cytosolic malate dehydrogenase;Malate dehydrogenase, cytoplasmic;MDH 1;MDH1

Molecular Weight

36483

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2140Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	344.7	11.88	3.4
Intra-Assay	2	18	174	6.16	3.5
Intra-Assay	3	18	239.2	10.6	4.4

Background

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.

Protocol

These standard curves of E2140Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480mU/mL	1.99	1.978	1.923
	1.967
240mU/mL	1.057	1.079	1.024
	1.102
120mU/mL	0.425	0.424	0.369
	0.423
60mU/mL	0.204	0.219	0.164
	0.234
30mU/mL	0.088	0.092	0.037
	0.097
0mU/mL	0.046	0.055	0
	0.064

Gene ID	24551
UniProt ID	O88989

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113272	E2140Ra	48T	$320		Add to Cart
BHE12113272	E2140Ra	96T	$458		Add to Cart

Rat Malate Dehydrogenase - Cytoplasmic - MDH1 ELISA Kit

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