Rat Leukemia Inhibitory Factor

Mfr.No.

E0143Ra

Sensitivity

0.053ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Leukemia Inhibitory Factor (also known as LIF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cholinergic neuronal differentiation factor;Leukemia inhibitory factor;LIF

Molecular Weight

22113

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0143Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.89	0.46	6.63
Intra-Assay	2	18	12.51	0.39	3.1
Intra-Assay	3	18	4.16	0.28	6.72

Background

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Protocol

These standard curves of E0143Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.341	2.287	2.227
	2.234
12ng/mL	1.448	1.341	1.28
	1.233
6ng/mL	0.675	0.735	0.674
	0.795
3ng/mL	0.428	0.441	0.38
	0.453
1.5ng/mL	0.283	0.304	0.243
	0.324
0ng/mL	0.061	0.061	0
	0.06

Gene ID	60584
UniProt ID	P17777

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111461	E0143Ra	48T	$320		Add to Cart
BHE12111461	E0143Ra	96T	$458		Add to Cart

Rat Leukemia Inhibitory Factor - LIF ELISA Kit

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