Rat KIM1 ELISA Kit PicoKine®

Mfr.No.

EK0882

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.029	0.055	0.105	0.271	0.482	0.760	1.200	1.838

Description

Rat KIM1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Havcr1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatitis A virus cellular receptor 1 homolog;HAVcr-1;Kidney injury molecule 1;KIM-1;T cell immunoglobulin and mucin domain-containing protein 1;TIMD-1;Havcr1;Kim1;

Molecular Weight

33964 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-V238

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat KIM1

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	87	272	938	86	293	1026
Standard deviation	3.82	20.67	59.09	3.95	23.14	84.13
CV (%)	4.4%	7.6%	7.9%	4.6%	7.9%	8.2%

Protein Function

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity). .

Background

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated intealization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	87	94	102	93	94	5.33	5.6%
Sample 2	272	238	277	253	260	15.54	5.9%
Sample 3	938	849	928	895	902	34.74	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	286934
Gene Name	HAVCR1
UniProt ID	O54947
Protein Name	Hepatitis A virus cellular receptor 1
Tissue Specificity	Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. .
Sequence	Belongs to the immunoglobulin superfamily. TIM family.

Applications	ELISA
Research Area	Adaptive Immunity,Host-Virus Interaction,Immune System Diseases,Immunology,Interspecies Interaction,Microbiology,Non-CD,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000675	EK0882	96 wells/kit	$399		Add to Cart

Rat KIM1 ELISA Kit PicoKine®

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