Rat JAM-C ELISA Kit PicoKine®

Mfr.No.

EK1841

Sensitivity

<5 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.039	0.108	0.186	0.321	0.599	1.017	1.586	2.200

Description

Rat JAM-C ELISA Kit PicoKine® (96 Tests). Quantitate Rat Jam3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAM-3; Jam3

Immunogen

Expression system for standard: NS0; Immunogen sequence: V32-N241

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat JAM-C

Subcellular Localization

extracellular space, Cell membrane, Single-pass type I membrane protein, desmosome

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	30	159	382	31	163	472
Standard deviation	1.29	7.31	21.77	1.92	10.27	34.93
CV (%)	4.3%	4.6%	5.7%	6.2%	6.3%	7.4%

Protein Function

May participate in cell-cell adhesion distinct from tight junctions.

Background

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 8q13. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	30	31	35	34	32	2.06	6.4%
Sample 2	159	171	172	173	168	5.67	3.3%
Sample 3	382	367	365	370	371	6.59	1.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	315509
Gene Name	Jam3
UniProt ID	Q68FQ2

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton/ECM,Platelets,Signal Transduction,Tags & Cell Markers,Vascular Inflammation,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001417	EK1841	96 wells/kit	$499		Add to Cart

Rat JAM-C ELISA Kit PicoKine®

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