Rat Interleukin 5 - IL-5 ELISA Kit

Mfr.No.

E0134Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 5 (also known as IL-5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

B-cell growth factor II;BCGF-II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;IL-5;Interleukin-5;T-cell replacing factor;TRF

Reactivity

Rat

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

15207

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.14ng/L

Background

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Gene ID	24497
UniProt ID	Q08125
Antigen	IL-5

Research Area

Immunology

Protocol

These standard curves of E0134Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.453	2.41	2.356
	2.367
600ng/L	1.567	1.452	1.398
	1.338
300ng/L	0.923	0.843	0.789
	0.763
150ng/L	0.497	0.455	0.401
	0.414
75ng/L	0.282	0.239	0.185
	0.196
0ng/L	0.056	0.054	0
	0.052

Precision

We measured random samples of E0134Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	309.8	19.4	6.26
Intra-Assay	2	18	652.47	25	3.83
Intra-Assay	3	18	139.29	7.6	5.46

Storage	2-8°C

Reference	An experimental model of asthma in rats using ovalbumin and lipopolysaccharide allergens Journal: Heliyon,Volume 5, Issue 11,2019,e02864

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111453	E0134Ra	48T	$320		Add to Cart
BHE12111453	E0134Ra	96T	$458		Add to Cart

Rat Interleukin 5 - IL-5 ELISA Kit

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