Rat Interleukin 3 - IL-3 ELISA Kit

Mfr.No.

E0131Ra

Sensitivity

0.47ng/L

Detection Range

1-400ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 3 (also known as IL-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Hematopoietic growth factor;IL-3;Interleukin-3;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor

Molecular Weight

18631

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0131Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	62.74	3.51	5.59
Intra-Assay	2	18	129.7	4.49	3.46
Intra-Assay	3	18	26.67	1.54	5.78

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Protocol

These standard curves of E0131Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.173	2.209	2.171
	2.246
120ng/L	1.5	1.48	1.442
	1.46
60ng/L	0.925	0.865	0.827
	0.805
30ng/L	0.538	0.515	0.477
	0.491
15ng/L	0.28	0.274	0.236
	0.269
0ng/L	0.035	0.038	0
	0.041

Gene ID	24495
UniProt ID	P04823

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111449	E0131Ra	48T	$320		Add to Cart
BHE12111449	E0131Ra	96T	$458		Add to Cart

Rat Interleukin 3 - IL-3 ELISA Kit

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