Rat Interleukin 15 - IL-15 ELISA Kit

Mfr.No.

E0113Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 15 (also known as IL-15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 15;IL15;IL-15;Interleukin-15

Reactivity

Rat

Detection Range

0.5-200ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

18804

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.32ng/L

Background

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (By similarity). In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (By similarity).

Gene ID	25670
UniProt ID	P97604
Antigen	IL15

Research Area

Immunology

Protocol

These standard curves of E0113Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.354	2.355	2.301
	2.355
60ng/L	1.562	1.538	1.485
	1.515
30ng/L	1.08	1.045	0.991
	1.009
15ng/L	0.778	0.767	0.714
	0.756
7.5ng/L	0.427	0.419	0.365
	0.41
0ng/L	0.053	0.053	0
	0.054

Precision

We measured random samples of E0113Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	71.5	3.19	4.5
Intra-Assay	2	18	35.6	1.91	5.4
Intra-Assay	3	18	7.7	0.52	6.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111431	E0113Ra	48T	$320		Add to Cart
BHE12111431	E0113Ra	96T	$458		Add to Cart

Rat Interleukin 15 - IL-15 ELISA Kit

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