Rat IL6ST ELISA Kit PicoKine®

Mfr.No.

EK1742

Sensitivity

<0.05 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Rat

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.008	0.329	0.516	0.817	1.102	1.336	1.568	1.839

Description

Rat IL6ST ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il6st in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-E618

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat IL6ST

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1254	4579	20188	1322	5546	21456
Standard deviation	66.46	290.3	847.9	99.15	321.7	1652.1
CV (%)	5.3%	6.1%	4.2%	7.5%	5.8%	7.7%

Protein Function

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, w

Background

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1254	1275	1273	1502	1326	101.94	7.6%
Sample 2	4597	4887	4558	4251	4573	225.32	4.9%
Sample 3	20188	20369	19931	19988	20119	173.03	0.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25205
Gene Name	Il6st
UniProt ID	P40190
Tissue Specificity	Found in hepatocytes, astrocytes, fibroblasts and endothelial cells.

Applications	ELISA
Research Area	Adapters,Apoptosis,Associated Proteins,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Type Markers,Cytokines,Hypertrophy,Immunology,Intracellular,Signal Transduction,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001384	EK1742	96 wells/kit	$499		Add to Cart

Rat IL6ST ELISA Kit PicoKine®

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